Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Zatloukal, K; Stumptner, C; Fuchsbichler, A; Heid, H; Schnoelzer, M; Kenner, L; Kleinert, R; Prinz, M G; Aguzzi, A; Denk, H (2002). p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases. American Journal of Pathology, 160(1):255-263.

Full text not available from this repository.

View at publisher

Abstract

Exposure of cells to stress, particularly oxidative stress, leads to misfolding of proteins and, if they are not refolded or degraded, to cytoplasmic protein aggregates. Protein aggregates are characteristic features of a variety of chronic toxic and degenerative diseases, such as Mallory bodies (MBs) in hepatocytes in alcoholic and non-alcoholic steatohepatitis, neurofibrillary tangles in neurons in Alzheimer's, and Lewy bodies in Parkinson's disease. Using 2D gel electrophoresis and mass spectrometry, we identified p62 as a novel MB component. p62 and cytokeratins (CKs) are major MB constituents; HSP 70, HSP 25, and ubiquitinated CKs are also present. These proteins characterize MBs as a prototype of disease-associated cytoplasmic inclusions generated by stress-induced protein misfolding. As revealed by transfection of tissue culture cells overexpressed p62 did not induce aggregation of regular CK filaments but selectively bound to misfolded and ubiquitinated CKs. The general role of p62 in the cellular response to misfolded proteins was substantiated by detection of p62 in other cytoplasmic inclusions, such as neurofibrillary tangles, Lewy bodies, Rosenthal fibers, intracytoplasmic hyaline bodies in hepatocellular carcinoma, and alpha1-antitrypsin aggregates. The presence of p62 along with other stress proteins and ubiquitin in cytoplasmic inclusions indicates deposition as aggregates as a third line of defense against misfolded proteins in addition to refolding and degradation.

Citations

Altmetrics

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2002
Deposited On:11 Feb 2008 12:27
Last Modified:27 Nov 2013 23:37
Publisher:Elsevier
ISSN:0002-9440
Publisher DOI:10.1016/S0002-9440(10)64369-6
Related URLs:http://ajp.amjpathol.org/cgi/content/abstract/160/1/255
PubMed ID:11786419

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page