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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-20976

Schneider, P; Krucker, T; Meyer, E; Ulmann-Schuler, A; Weber, B; Stampanoni, M; Müller, R (2009). Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica. Microscopy Research and Technique, 72(9):690-701.

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Abstract

Recent evidence suggests a close functional relationship between osteogenesis and angiogenesis as well as between bone remodeling and bone vascularization. Consequently, there is a need for visual inspection and quantitative analysis of the bone vasculature. We therefore adapted and implemented two different vascular corrosion casting (VCC) protocols using a polyurethane-based casting resin in mice for a true three-dimensional (3D), direct, and simultaneous measurement of bone tissue and vascular morphology by micro-computed tomography (microCT). For assessment of vascular replicas at the level of capillaries, a vascular contrast perfusion (VCP) protocol was devised using a contrast modality based on a barium sulfate suspension in conjunction with synchrotron radiation (SR) microCT. The vascular morphology quantified using the VCP protocol was compared quantitatively with the results of a previously established method, where the vascular network of cortical bone was derived indirectly from cortical porosity. The presented VCC and VCP protocols have the potential of serving as a valuable method for concomitant 3D quantitative morphometry of the bone tissue and its vasculature.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
04 Faculty of Medicine > Institute of Biomedical Engineering
DDC:570 Life sciences; biology
170 Ethics
610 Medicine & health
Language:English
Date:September 2009
Deposited On:30 Sep 2009 13:38
Last Modified:03 Dec 2013 07:40
Publisher:Wiley-Blackwell
ISSN:1059-910X
Additional Information:The definitive version is available at www.blackwell-synergy.com
Publisher DOI:10.1002/jemt.20720
PubMed ID:19360841
Citations:Web of Science®. Times Cited: 16
Google Scholar™
Scopus®. Citation Count: 17

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