Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-20976
Schneider, P; Krucker, T; Meyer, E; Ulmann-Schuler, A; Weber, B; Stampanoni, M; Müller, R (2009). Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica. Microscopy Research and Technique, 72(9):690-701.
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Recent evidence suggests a close functional relationship between osteogenesis and angiogenesis as well as between bone remodeling and bone vascularization. Consequently, there is a need for visual inspection and quantitative analysis of the bone vasculature. We therefore adapted and implemented two different vascular corrosion casting (VCC) protocols using a polyurethane-based casting resin in mice for a true three-dimensional (3D), direct, and simultaneous measurement of bone tissue and vascular morphology by micro-computed tomography (microCT). For assessment of vascular replicas at the level of capillaries, a vascular contrast perfusion (VCP) protocol was devised using a contrast modality based on a barium sulfate suspension in conjunction with synchrotron radiation (SR) microCT. The vascular morphology quantified using the VCP protocol was compared quantitatively with the results of a previously established method, where the vascular network of cortical bone was derived indirectly from cortical porosity. The presented VCC and VCP protocols have the potential of serving as a valuable method for concomitant 3D quantitative morphometry of the bone tissue and its vasculature.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Pharmacology and Toxicology|
04 Faculty of Medicine > Institute of Biomedical Engineering
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||30 Sep 2009 15:38|
|Last Modified:||10 Dec 2012 13:30|
|Additional Information:||The definitive version is available at www.blackwell-synergy.com|
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