Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-21045
Kim, S; Gailite, I; Moussian, B; Luschnig, S; Goette, M; Fricke, K; Honemann-Capito, M; Grubmüller, H; Wodarz, A (2009). Kinase-activity-independent functions of atypical protein kinase C in Drosophila. Journal of Cell Science, 122(PT 20):3759-3771.
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Polarity of many cell types is controlled by a protein complex consisting of Bazooka/PAR-3 (Baz), PAR-6 and atypical protein kinase C (aPKC). In Drosophila, the Baz-PAR-6-aPKC complex is required for the control of cell polarity in the follicular epithelium, in ectodermal epithelia and neuroblasts. aPKC is the main signaling component of this complex that functions by phosphorylating downstream targets, while the PDZ domain proteins Baz and PAR-6 control the subcellular localization and kinase activity of aPKC. We compared the mutant phenotypes of an aPKC null allele with those of four novel aPKC alleles harboring point mutations that abolish the kinase activity or the binding of aPKC to PAR-6. We show that these point alleles retain full functionality in the control of follicle cell polarity, but produce strong loss-of-function phenotypes in embryonic epithelia and neuroblasts. Our data, combined with molecular dynamics simulations, show that the kinase activity of aPKC and its ability to bind PAR-6 are only required for a subset of its functions during development, revealing tissue-specific differences in the way that aPKC controls cell polarity.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Date:||29 September 2009|
|Deposited On:||13 Oct 2009 10:51|
|Last Modified:||27 Nov 2013 22:44|
|Publisher:||Company of Biologists|
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