Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-21117

Haas, C; Muheim, C; Kratzer, A; Bär, W; Maake, C (2009). mRNA profiling for the identification of sperm and seminal plasma. Forensic Science International: Genetics Supplement Series, 2(1):534-535.

[img]
Preview
Accepted Version
PDF
1MB

View at publisher

Abstract

mRNA profiling is a promising new method for the identification of body fluids from biological stains. In this study we aimed to establish a multiplex RT-PCR protocol for the detection and differentiation of sperm and seminal plasma. The Agilent Bioanalyzer and Nanodrop spectrophotometer were shown not to be suitable for assessing RNA quality and quantity of forensic stains. Semen specificity of the mRNA markers was successfully confirmed with singleplex PCR. Our data indicated that semen samples down to 0.1 ml and up to 20-year-old could be identified with mRNA profiling. With the semen multiplex, including 2 sperm markers (PRM1, PRM2) and 2 novel seminal plasma markers (SEMG1, PSA), samples from azoospermic men (absence of sperm in semen) are clearly distinguishable from those of normozoospermic men (having a normal sperm production). We think that our multiplex RT-PCR protocol is a reliable and sensitive method for the identification of semen in forensic samples.

Citations

Altmetrics

Downloads

260 downloads since deposited on 06 Oct 2009
20 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Legal Medicine
04 Faculty of Medicine > Institute of Anatomy
DDC:570 Life sciences; biology
340 Law
610 Medicine & health
Language:English
Date:2009
Deposited On:06 Oct 2009 12:18
Last Modified:23 Nov 2012 13:09
Publisher:Elsevier
ISSN:1875-175X
Publisher DOI:10.1016/j.fsigss.2009.08.109

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page