UZH-Logo

Maintenance Infos

Cell-type-specific TEV protease cleavage reveals Cohesin functions in Drosophila neurons


Pauli, A; Althoff, F; Oliveira, R A; Heidmann, S; Schuldiner, O; Lehner, C F; Dickson, B J; Nasmyth, K (2008). Cell-type-specific TEV protease cleavage reveals Cohesin functions in Drosophila neurons. Developmental Cell, 14(2):239-251.

Abstract

Cohesin is a highly conserved multi-subunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin’s dissociation from chromosomes and triggers sister chromatid disjunction. To investigate cohesin’s function in post-mitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in post-mitotic neurons is lethal. In mushroom body neurons it causes defects in axon pruning while in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in non-dividing cells, and also introduce a powerful new tool to investigate protein function in metazoa.

Cohesin is a highly conserved multi-subunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin’s dissociation from chromosomes and triggers sister chromatid disjunction. To investigate cohesin’s function in post-mitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in post-mitotic neurons is lethal. In mushroom body neurons it causes defects in axon pruning while in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in non-dividing cells, and also introduce a powerful new tool to investigate protein function in metazoa.

Citations

133 citations in Web of Science®
142 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

184 downloads since deposited on 04 Mar 2008
15 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Drosophila; cohesin complex; TEV protease; neurons; polytene chromosomes
Language:English
Date:12 February 2008
Deposited On:04 Mar 2008 09:11
Last Modified:05 Apr 2016 12:22
Publisher:Elsevier
ISSN:1534-5807
Publisher DOI:10.1016/j.devcel.2007.12.009
PubMed ID:18267092
Permanent URL: http://doi.org/10.5167/uzh-2272

Download

[img]
Preview
Content: Accepted Version
Filetype: PDF
Size: 3MB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations