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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-237

Stainier, D Y; Fouquet, B; Chen, J N; Warren, K S; Weinstein, B M; Meiler, S E; Mohideen, M A; Neuhauss, S C F; Solnica-Krezel, L; Schier, A F; Zwartkruis, F J T; Stemple, D L; Malicki, J; Driever, W; Fishman, M C (1996). Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo. Development, 123(UNSPECIFIE):285-292.

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Abstract

As part of a large-scale mutagenesis screen of the zebrafish genome, we have identified 58 mutations that affect the formation and function of the cardiovascular system. The cardiovascular system is particularly amenable for screening in the transparent zebrafish embryo because the heart and blood vessels are prominent and their function easily examined. We have classified the mutations affecting the heart into those that affect primarily either morphogenesis or function. Nine mutations clearly disrupt the formation of the heart. cloche deletes the endocardium. In cloche mutants, the myocardial layer forms in the absence of the endocardium but is dysmorphic and exhibits a weak contractility. Two loci, miles apart and bonnie and clyde, play a critical role in the fusion of the bilateral tubular primordia. Three mutations lead to an abnormally large heart and one to the formation of a diminutive, dysmorphic heart. We have found no mutation that deletes the myocardial cells altogether, but one, pandora, appears to eliminate the ventricle selectively. Seven mutations interfere with vascular integrity, as indicated by hemorrhage at particular sites. In terms of cardiac function, one large group exhibits a weak beat. In this group, five loci affect both chambers and seven a specific chamber (the atrium or ventricle). For example, the weak atrium mutation exhibits an atrium that becomes silent but has a normally beating ventricle. Seven mutations affect the rhythm of the heart causing, for example, a slow rate, a fibrillating pattern or an apparent block to conduction. In several other mutants, regurgitation of blood flow from ventricle to atrium is the most prominent abnormality, due either to the absence of valves or to poor coordination between the chambers with regard to the timing of contraction. The mutations identified in this screen point to discrete and critical steps in the formation and function of the heart and vasculature.

Item Type:Journal Article, refereed
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:01 December 1996
Deposited On:11 Feb 2008 13:13
Last Modified:27 Nov 2013 20:19
Publisher:Company of Biologists
ISSN:0950-1991
Related URLs:http://dev.biologists.org/cgi/reprint/123/1/285
PubMed ID:9007248
Citations:Web of Science®. Times Cited: 308
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