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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-24156

Schmidt, S; Blom, J F; Pernthaler, J; Berg, G; Baldwin, A; Mahenthiralingam, E; Eberl, L (2009). Production of the antifungal compound pyrrolnitrin is quorum sensing-regulated in members of the Burkholderia cepacia complex. Environmental Microbiology, 11(6):1422-1437.

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Abstract

Members of the genus Burkholderia are known for their ability to suppress soil-borne fungal pathogens by the production of various antibiotic compounds. In this study we investigated the role of N-acylhomoserine lactone (AHL)-dependent quorum sensing (QS) in the expression of antifungal traits. Using a quorum quenching approach, that is, by heterologous expression of the Bacillus sp. AiiA lactonase, we show that expression of antifungal activities is AHL-dependent in the large majority of the investigated strains belonging to various Burkholderia species. We demonstrate that in certain strains of Burkholderia ambifaria, Burkholderia pyrrocinia and Burkholderia lata, one of the QS-regulated antifungal agents is pyrrolnitrin (prn), a common broad-spectrum antibiotic that is also produced by some Pseudomonas and Serratia species. To investigate the underlying molecular mechanisms of AHL-dependent prn production in better detail, we inactivated the AHL synthase cepI as well as cepR, which encodes the cognate AHL receptor protein, in B. lata 383. Both QS mutants no longer produced prn as assessed by gas chromatography-mass spectrometry analysis and as a consequence were unable to inhibit growth of Rhizoctonia solani. Using fusions of the lacZ gene to the promoter of the prnABCD operon, which directs the synthesis of prn, we demonstrate that expression of prn is positively regulated by CepR at the level of transcription.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Plant Biology
DDC:580 Plants (Botany)
Date:2009
Deposited On:04 Dec 2009 06:11
Last Modified:27 Nov 2013 16:32
Publisher:Wiley-Blackwell
ISSN:1462-2912
Additional Information:Free access at DOI
Publisher DOI:10.1111/j.1462-2920.2009.01870.x
PubMed ID:19220396
Citations:Web of Science®. Times Cited: 27
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Scopus®. Citation Count: 29

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