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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-24163

Malott, R J; O'Grady, E P; Toller, J; Inhülsen, S; Eberl, L; Sokol, P A (2009). A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation. Journal of Bacteriology, 191(8):2447-2460.

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Abstract

Burkholderia cenocepacia utilizes quorum sensing to control gene expression, including the expression of genes involved in virulence. In addition to CepR and CciR, a third LuxR homolog, CepR2, was found to regulate gene expression and virulence factor production. All B. cenocepacia strains examined contained this orphan LuxR homolog, which was not associated with an adjacent N-acyl-homoserine lactone synthase gene. Expression of cepR2 was negatively autoregulated and was negatively regulated by CciR in strain K56-2. Microarray analysis and quantitative reverse transcription-PCR determined that CepR2 did not influence expression of cepIR or cciIR. However, in strain K56-2, CepR2 negatively regulated expression of several known quorum-sensing-controlled genes, including genes encoding zinc metalloproteases. CepR2 exerted positive and negative regulation on genes on three chromosomes, including strong negative regulation of a gene cluster located adjacent to cepR2. In strain H111, which lacks the CciIR quorum-sensing system, CepR2 positively regulated pyochelin production by controlling transcription of one of the operons required for the biosynthesis of the siderophore in an N-acyl-homoserine lactone-independent manner. CepR2 activation of a luxI promoter was demonstrated in a heterologous Escherichia coli host, providing further evidence that CepR2 can function in the absence of signaling molecules. This study demonstrates that the orphan LuxR homolog CepR2 contributes to the quorum-sensing regulatory network in two distinct strains of B. cenocepacia.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Plant Biology
DDC:580 Plants (Botany)
Date:6 February 2009
Deposited On:04 Dec 2009 09:05
Last Modified:27 Nov 2013 19:20
Publisher:American Society for Microbiology
ISSN:0021-9193
Additional Information:Copyright: American Society for Microbiology
Publisher DOI:10.1128/JB.01746-08
PubMed ID:19201791
Citations:Web of Science®. Times Cited: 26
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Scopus®. Citation Count: 27

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