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Cross-species utility of microsatellite markers in trichostrongyloid nematodes


Temperley, N D; Webster, L M I; Adam, A; Keller, L F; Johnson, P C D (2009). Cross-species utility of microsatellite markers in trichostrongyloid nematodes. The Journal of Parasitology, 95(2):487-489.

Abstract

The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.

The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:2009
Deposited On:08 Mar 2010 17:57
Last Modified:05 Apr 2016 13:34
Publisher:American Society of Parasitologists
ISSN:0022-3395
Publisher DOI:10.1645/GE-1624.1
PubMed ID:18817456
Permanent URL: http://doi.org/10.5167/uzh-24490

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