Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-249
Fitzli, D; Stoeckli, E T; Kunz, S; Siribour, K; Rader, C; Kunz, B; Kozlov, S V; Buchstaller, A; Lane, R P; Suter, D M; Dreyer, W J; Sonderegger, P (2000). A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons. Journal of Cell Biology, 149(4):951-968.
An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Biochemistry|
07 Faculty of Science > Institute of Biochemistry
07 Faculty of Science > Institute of Molecular Life Sciences
|DDC:||570 Life sciences; biology|
|Date:||15 May 2000|
|Deposited On:||11 Feb 2008 13:14|
|Last Modified:||27 Nov 2013 23:53|
|Publisher:||Rockefeller University Press|
|Free access at:||Official URL. An embargo period may apply.|
|Citations:||Web of Science®. Times Cited: 48|
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