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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-25089

Carranza, P; Hartmann, I; Lehner, A; Stephan, R; Gehrig, P; Grossmann, J; Barkow-Oesterreicher, S; Roschitzki, B; Eberl, L; Riedel, K (2009). Proteomic profiling of Cronobacter turicensis 3032, a food-borne opportunistic pathogen. Proteomics, 9(13):3564-3579.

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Abstract

Members of the genus Cronobacter are opportunistic pathogens for neonates and are often associated with contaminated milk powder formulas. At present little is known about the virulence mechanisms or the natural reservoir of these organisms. The proteome of Cronobacter turicensis 3032, which has recently caused two deaths, was mapped aiming at a better understanding of physiology and putative pathogenic traits of this clinical isolate. Our analyses of extracellular, surface-associated and whole-cell proteins by two complementary proteomics approaches, 1D-SDS-PAGE combined with LC-ESI-MS/MS and 2D-LC-MALDI-TOF/TOF MS, lead to the identification of 832 proteins corresponding to a remarkable 19% of the theoretically expressed protein complement of C. turicensis. The majority of the identified proteins are involved in central metabolic pathways, translation, protein folding and stability. Several putative virulence factors, whose expressions were confirmed by phenotypic assays, could be identified: a macrophage infectivity potentiator involved in C. turicensis persistence in host cells, a superoxide dismutase protecting the pathogen against reactive oxygen species and an enterobactin-receptor protein for the uptake of siderophore-bound iron. Most interestingly, a chitinase and a metalloprotease that might act against insects and fungi but no casein hydrolysing enzymes were found, suggesting that there is an environmental natural habitat of C. turicensis 3032.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Functional Genomics Center Zurich
07 Faculty of Science > Institute of Plant Biology
05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
08 University Research Priority Programs > Systems Biology / Functional Genomics
DDC:570 Life sciences; biology
580 Plants (Botany)
610 Medicine & health
Language:English
Date:2009
Deposited On:02 Dec 2009 15:12
Last Modified:27 Nov 2013 18:48
Publisher:Wiley-Blackwell
ISSN:1615-9853
Publisher DOI:10.1002/pmic.200900016
PubMed ID:19609963
Citations:Web of Science®. Times Cited: 7
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Scopus®. Citation Count: 8

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