UZH-Logo

Maintenance Infos

A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms


Gmür, R; Lüthi-Schaller, H (2007). A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms. Journal of Microbiological Methods, 69(2):402-405.

Abstract

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.

Citations

15 citations in Web of Science®
13 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

114 downloads since deposited on 16 Jun 2008
31 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2007
Deposited On:16 Jun 2008 14:54
Last Modified:05 Apr 2016 12:23
Publisher:Elsevier
ISSN:0167-7012
Publisher DOI:10.1016/j.mimet.2006.12.012
PubMed ID:17258338
Permanent URL: http://doi.org/10.5167/uzh-2566

Download

[img]
Preview
Content: Accepted Version
Filetype: PDF
Size: 1MB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations