UZH-Logo

The alternative sigma factor sigma(L) of L. monocytogenes promotes growth under diverse environmental stresses


Raimann, E; Schmid, B; Stephan, R; Tasara, T (2009). The alternative sigma factor sigma(L) of L. monocytogenes promotes growth under diverse environmental stresses. Foodborne Pathogens and Disease, 6(5):583-591.

Abstract

Listeria monocytogenes can cause sporadic cases and outbreaks of serious illnesses due to consumption of contaminated food products The challenge of this food-borne pathogen can in part be attributed to its remarkable stress tolerance. L. monocytogenes organisms efficiently adapt and sometimes proliferate despite exposure to low temperatures, low pH and elevated salt (NaCl) concentrations. To gain insights into some of the underlying adaptive mechanisms, the contribution of alternative sigma factor σL to counter such environmental stresses was investigated by quantitative RT-PCR of sigL gene transcripts and phenotypic growth evaluation of a sigL deletion mutant. Moreover, transcripts of selected σL regulated genes (oppA, cspD and clpP) were quantified in cold and NaCl stress adapted cells of a ΔsigL and the wild type strain. We found that sigL transcripts are variably but significantly induced by exposure of L. monocytogenes to the three different growth stress conditions. The growth of the ΔsigL strain was significantly impaired in comparison to the wild type under all stress conditions applied. Additionally, transcripts of oppA were more than 50-fold reduced in cold adapted ΔsigL cells compared to the wild type cells. These results suggest an important role of σL dependent pathways in L. monocytogenes adaptation against low temperatures, low pH and elevated salt (NaCl) concentrations

Listeria monocytogenes can cause sporadic cases and outbreaks of serious illnesses due to consumption of contaminated food products The challenge of this food-borne pathogen can in part be attributed to its remarkable stress tolerance. L. monocytogenes organisms efficiently adapt and sometimes proliferate despite exposure to low temperatures, low pH and elevated salt (NaCl) concentrations. To gain insights into some of the underlying adaptive mechanisms, the contribution of alternative sigma factor σL to counter such environmental stresses was investigated by quantitative RT-PCR of sigL gene transcripts and phenotypic growth evaluation of a sigL deletion mutant. Moreover, transcripts of selected σL regulated genes (oppA, cspD and clpP) were quantified in cold and NaCl stress adapted cells of a ΔsigL and the wild type strain. We found that sigL transcripts are variably but significantly induced by exposure of L. monocytogenes to the three different growth stress conditions. The growth of the ΔsigL strain was significantly impaired in comparison to the wild type under all stress conditions applied. Additionally, transcripts of oppA were more than 50-fold reduced in cold adapted ΔsigL cells compared to the wild type cells. These results suggest an important role of σL dependent pathways in L. monocytogenes adaptation against low temperatures, low pH and elevated salt (NaCl) concentrations

Citations

27 citations in Web of Science®
26 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 11 Jan 2010
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:11 Jan 2010 16:52
Last Modified:05 Apr 2016 13:38
Publisher:Mary Ann Liebert
ISSN:1535-3141
Publisher DOI:10.1089/fpd.2008.0248
PubMed ID:19422306
Permanent URL: http://doi.org/10.5167/uzh-25697

Download

[img]
Filetype: PDF - Registered users only
Size: 1MB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations