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In vitro inhibition activity of different bacteriocin-producing E. coli against Salmonella strains isolated from clinical cases


Zihler, A; Le Blay, G; de Wouters, T; Lacroix, C; Braegger, C; Lehner, A; Tischler, P; Rattei, T; Haechler, H; Stephan, R (2009). In vitro inhibition activity of different bacteriocin-producing E. coli against Salmonella strains isolated from clinical cases. Letters in Applied Microbiology, 49(1):31-38.

Abstract

Aims: To compare in vitro the inhibitory activity of four bacteriocin producing E. coli to a well-characterized panel of Salmonella strains, recently isolated from clinical cases in Switzerland.
Methods and Results: A panel of 68 non-typhoidal Salmonella strains was characterized by PFGE analysis and susceptibility to antibiotics. The majority of tested strains were genetically different, with 40% resistant to at least one antibiotic. E. coli Mcc24 showed highest in vitro activity against Salmonella (100%, microcin 24), followed by E. coli L1000 (94%, microcin B17), E. coli 53 (49%, colicin H) and E. coli 52 (21%, colicin G) as revealed using a cross-streak activity assay.
Conclusion: E. coli Mcc24, a genetically modified organism producing microcin 24, and E. coli L1000, a natural strain isolated from human feces carrying the mcb-operon for microcin B17-production, were the most effective strains in inhibiting in vitro both antibiotic resistant and sensitive Salmonella isolates.
Significance and Impact of the Study: Due to an increasing prevalence of antibiotic resistant Salmonella strains, alternative strategies to fight these foodborne pathogens are needed. E. coli L1000 appears to be a promising candidate in view of developing biotechnological alternatives to antibiotics against Salmonella infections.

Aims: To compare in vitro the inhibitory activity of four bacteriocin producing E. coli to a well-characterized panel of Salmonella strains, recently isolated from clinical cases in Switzerland.
Methods and Results: A panel of 68 non-typhoidal Salmonella strains was characterized by PFGE analysis and susceptibility to antibiotics. The majority of tested strains were genetically different, with 40% resistant to at least one antibiotic. E. coli Mcc24 showed highest in vitro activity against Salmonella (100%, microcin 24), followed by E. coli L1000 (94%, microcin B17), E. coli 53 (49%, colicin H) and E. coli 52 (21%, colicin G) as revealed using a cross-streak activity assay.
Conclusion: E. coli Mcc24, a genetically modified organism producing microcin 24, and E. coli L1000, a natural strain isolated from human feces carrying the mcb-operon for microcin B17-production, were the most effective strains in inhibiting in vitro both antibiotic resistant and sensitive Salmonella isolates.
Significance and Impact of the Study: Due to an increasing prevalence of antibiotic resistant Salmonella strains, alternative strategies to fight these foodborne pathogens are needed. E. coli L1000 appears to be a promising candidate in view of developing biotechnological alternatives to antibiotics against Salmonella infections.

Citations

9 citations in Web of Science®
9 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:15 Jan 2010 16:12
Last Modified:05 Apr 2016 13:38
Publisher:Wiley-Blackwell
ISSN:0266-8254
Publisher DOI:10.1111/j.1472-765X.2009.02614.x
PubMed ID:19413755

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