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Sexing of turkey poults by Fourier transform infrared spectroscopy


Steiner, G; Bartels, T; Krautwald-Junghanns, M E; Boos, A; Koch, E (2010). Sexing of turkey poults by Fourier transform infrared spectroscopy. Analytical and Bioanalytical Chemistry, 396(1):465-470.

Abstract

Fourier transform infrared (FT-IR) spectroscopy was used to probe the molecular composition of germinal cells and to identify the gender of turkey poults. Germinal cells obtained from a feather pulp were characterized by FT-IR micro spectroscopy. The sample set consisted of growing contour feathers from 23 male and 23 female turkey poults. Significant spectral variations were observed in the range between 1,000 and 1,250 cm(-1). The spectra of male turkey poults exhibit a significantly higher content of RNA than those of female turkeys. Spectral classification was performed by a non-supervised method based on the principal component analysis. An evaluation of the first and third PCs led to a classification of female and male poults with an accuracy of more than 95%.

Fourier transform infrared (FT-IR) spectroscopy was used to probe the molecular composition of germinal cells and to identify the gender of turkey poults. Germinal cells obtained from a feather pulp were characterized by FT-IR micro spectroscopy. The sample set consisted of growing contour feathers from 23 male and 23 female turkey poults. Significant spectral variations were observed in the range between 1,000 and 1,250 cm(-1). The spectra of male turkey poults exhibit a significantly higher content of RNA than those of female turkeys. Spectral classification was performed by a non-supervised method based on the principal component analysis. An evaluation of the first and third PCs led to a classification of female and male poults with an accuracy of more than 95%.

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8 citations in Web of Science®
9 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
Dewey Decimal Classification:570 Life sciences; biology
Date:20 November 2010
Deposited On:25 Jan 2010 17:47
Last Modified:05 Apr 2016 13:38
Publisher:Springer
ISSN:1618-2642
Additional Information:The original publication is available at www.springerlink.com
Publisher DOI:https://doi.org/10.1007/s00216-009-3273-z
PubMed ID:19936721
Permanent URL: https://doi.org/10.5167/uzh-25749

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