Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-25754

Titz, A; Butschi, A; Henrissat, B; Fan, Y Y; Hennet, T; Razzazi-Fazeli, E; Hengartner, M O; Wilson, I B H; Kuenzler, M; Aebi, M (2009). Molecular basis for galactosylation of core fucose residues in invertebrates: Identification of Caenorhabditis elegans N-glycan core {alpha}1,6-fucoside {beta}1,4-galactosyltransferase GALT-1 as a member of a novel glycosyltransferase family. Journal of Biological Chemistry, 284(52):36223-36233.

[img]
Preview
Accepted Version
PDF
4MB

View at publisher

Abstract

Galectin CGL2 from the ink cap mushroom Coprinopsis cinerea displays toxicity towards the model nematode Caenorhabditis elegans. A mutation in a putative glycosyltransferase-encoding gene resulted in a CGL2-resistant C. elegans strain characterized by N-glycans lacking the beta1,4-galactoside linked to the alpha1,6-linked core fucose. Expression of the corresponding GALT-1 protein in insect cells was used to demonstrate a manganese dependent galactosyltransferase activity. In vitro, the GALT-1 enzyme showed strong selectivity for acceptors with alpha1,6-linked N-glycan core fucosides and required Golgi-dependent modifications on the oligosaccharide antennae for optimal synthesis of the Galbeta1,4Fuc structure. Phylogenetic analysis of the GALT-1 protein sequence identified a novel glycosyltransferase family (GT92) with members widespread among eukarya but absent in mammals.

Citations

21 citations in Web of Science®
22 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

107 downloads since deposited on 16 Dec 2009
35 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
07 Faculty of Science > Institute of Molecular Life Sciences
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:December 2009
Deposited On:16 Dec 2009 12:56
Last Modified:28 Nov 2013 00:31
Publisher:American Society for Biochemistry and Molecular Biology
ISSN:0021-9258
Additional Information:This research was originally published in Titz, A; Butschi, A; Henrissat, B; Fan, Y Y; Hennet, T; Razzazi-Fazeli, E; Hengartner, M O; Wilson, I B H; Kuenzler, M; Aebi, M (2009). Molecular basis for galactosylation of core fucose residues in invertebrates: Identification of Caenorhabditis elegans N-glycan core {alpha}1,6-fucoside {beta}1,4-galactosyltransferase GALT-1 as a member of a novel glycosyltransferase family. Journal of Biological Chemistry, 284(52):36223-36233. © the American Society for Biochemistry and Molecular Biology.
Publisher DOI:10.1074/jbc.M109.058354
PubMed ID:19858195

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page