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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-26240

Zini, E; Franchini, M; Guscetti, F; Osto, M; Kaufmann, K; Ackermann, M; Lutz, T A; Reusch, C E (2009). Assessment of six different collagenase-based methods to isolate feline pancreatic islets. Research in Veterinary Science, 87(3):367-372.

Accepted Version


Isolation of pancreatic islets is necessary to study the molecular mechanisms underlying beta-cell demise in diabetic cats. Six collagenase-based methods of isolation were compared in 10 cat pancreata, including single and double course of collagenase, followed or not by Ficoll centrifugation or accutase, and collagenase plus accutase. Morphometric analysis was performed to measure the relative area of islet and exocrine tissue. Islet specific mRNA transcripts were quantified in isolates by real-time PCR. The single and double course of collagenase digestion was successful in each cat and provided similar islet-to-exocrine tissue ratio. Quantities of insulin mRNA did not differ between the two methods. However, on histological examination either method yielded only approximately 2% of pure islets. The other methods provided disrupted islets or insufficient samples in 1-7 cats. Although pancreas digestion with single and double course of collagenase was superior, further studies are needed to improve islet isolation in cats.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Institute of Veterinary Pathology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals > Clinic for Small Animal Internal Medicine
05 Vetsuisse Faculty > Institute of Virology
DDC:570 Life sciences; biology
630 Agriculture
Deposited On:18 Jan 2010 15:11
Last Modified:27 Nov 2013 17:08
Publisher DOI:10.1016/j.rvsc.2009.03.017
PubMed ID:19386336
Citations:Web of Science®. Times Cited: 1
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Scopus®. Citation Count: 3

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