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Formation of supported lipid bilayers on indium tin oxide for dynamically-patterned membrane-functionalized microelectrode arrays


Kumar, K; Tang, C S; Rossetti, F F; Textor, M; Keller, B; Vörös, J; Reimhult, E (2009). Formation of supported lipid bilayers on indium tin oxide for dynamically-patterned membrane-functionalized microelectrode arrays. Lab on a Chip, 9(5):718-725.

Abstract

Supported lipid bilayers (SLBs) are ideal platforms for the study of membrane proteins and function. Assembly of functional SLBs in an array format would lead to a breakthrough in high-throughput screening of membrane-associated processes, e.g., drugs binding to transmembrane proteins. We report the formation of SLBs from the rupture of anionic vesicles in the presence of Ca(2+) ions on ITO-coated surfaces and characterise the assembly and SLB properties. Furthermore, the formation, manipulation and regeneration of SLBs adsorbed on ITO microelectrode array spots using an electric potential switch are demonstrated. This platform enables addressable assembly and the study of electrochemically mediated membrane processes in a microarray format which can be regenerated in situ.

Supported lipid bilayers (SLBs) are ideal platforms for the study of membrane proteins and function. Assembly of functional SLBs in an array format would lead to a breakthrough in high-throughput screening of membrane-associated processes, e.g., drugs binding to transmembrane proteins. We report the formation of SLBs from the rupture of anionic vesicles in the presence of Ca(2+) ions on ITO-coated surfaces and characterise the assembly and SLB properties. Furthermore, the formation, manipulation and regeneration of SLBs adsorbed on ITO microelectrode array spots using an electric potential switch are demonstrated. This platform enables addressable assembly and the study of electrochemically mediated membrane processes in a microarray format which can be regenerated in situ.

Citations

21 citations in Web of Science®
22 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Language:English
Date:2009
Deposited On:22 Dec 2009 13:48
Last Modified:05 Apr 2016 13:41
Publisher:Royal Society of Chemistry
ISSN:1473-0189
Publisher DOI:10.1039/b814281e
PubMed ID:19224023

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