Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-27027
McDonald, S A; Marone, F; Hintermüller, C; Bensadoun, J C; Aebischer, P; Stampanoni, M (2009). High-throughput, high-resolution X-ray phase contrast tomographic microscopy for visualisation of soft tissue. Journal of Physics: Conference Series, 186:012043.
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The use of conventional absorption based X-ray microtomography can become limited for samples showing only very weak absorption contrast. However, a wide range of samples studied in biology and materials science can produce significant phase shifts of the Xray beam, and thus the use of the phase signal can provide substantially increased contrast and therefore new and otherwise inaccessible information. The application of two approaches for high-throughput, high-resolution X-ray phase contrast tomography, both available on the TOMCAT beamline of the SLS, is illustrated. Differential Phase Contrast (DPC) imaging uses a grating interferometer and a phase-stepping technique. It has been integrated into the beamline environment on TOMCAT in terms of the fast acquisition and reconstruction of data and the availability to scan samples within an aqueous environment. The second phase contrast approach is a modified transfer of intensity approach that can yield the 3D distribution of the phase (refractive index) of a weakly absorbing object from a single tomographic dataset. These methods are being used for the evaluation of cell integrity in 3D, with the specific aim of following and analyzing progressive cell degeneration to increase knowledge of the mechanistic events of neurodegenerative disorders such as Parkinson’s disease.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Biomedical Engineering|
|Dewey Decimal Classification:||170 Ethics
610 Medicine & health
|Deposited On:||22 Jan 2010 15:49|
|Last Modified:||27 Nov 2013 18:43|
|Publisher:||Institute of Physics Publishing|
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