Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-27153
Templin, C; Grote, K; Schledzewski, K; Ghadri, J R; Schnabel, S; Napp, L C; Schieffer, B; Kurzen, H; Goerdt, S; Landmesser, U; Koenen, W; Faulhaber, J (2009). Ex vivo expanded haematopoietic progenitor cells improve dermal wound healing by paracrine mechanisms. Experimental Dermatology, 18(5):445-453.
View at publisher
BACKGROUND: Although dermal wounds are common, treatment remains limited and largely ineffective. Recent studies suggest that therapeutic application of progenitor cells is useful for tissue regeneration. OBJECTIVE: We here investigated the effects exerted by the recently characterized immortalized haematopoietic progenitor cell line DKmix and their conditioned medium in a murine wound healing model. METHODS AND RESULTS: Injection of both DKmix cells and their conditioned medium accelerated wound repair between days 3 and 10 compared with PBS-injected control mice (n = 8, P < 0.01 DKmix cells vs control, P < 0.01 conditioned medium vs control at day 6). The treated groups exhibited more CD31(+)-capillaries at day 6 after injury compared with the control group (n = 4, P < 0.01 DKmix cells vs control, P < 0.001 conditioned medium vs control), whereas there was no change in infiltrated CD68(+) macrophages. Conditioned medium of DKmix cells induced tube formation of human endothelial cells in Matrigel assays (n = 4-6, P < 0.05 conditioned medium vs control) as well as migration (n = 4, P < 0.01 conditioned medium vs control) and proliferation of murine 3T3 fibroblasts (n = 5, P < 0.05 conditioned medium vs control). Abundant levels of matrix metalloproteinase -2 and -9 in the supernatants were detected. Protein arrays of the supernatants revealed a strong secretion of cytokines and growth factors, such as monocyte chemoatractant protein-1 and GM-CSF from DKmix cells. CONCLUSION: DKmix cells improve skin-substitute wound healing by promoting angiogenesis as well as migration and proliferation of fibroblasts. These data suggest that immortalized haematopoietic progenitor cells significantly improve dermal wound healing by paracrine effects.
99 downloads since deposited on 13 Jan 2010
47 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine
04 Faculty of Medicine > University Hospital Zurich > Clinic for Cardiology
|Dewey Decimal Classification:||610 Medicine & health|
|Deposited On:||13 Jan 2010 09:03|
|Last Modified:||27 Nov 2013 20:52|
|Additional Information:||The definitive version is available at www.blackwell-synergy.com|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page