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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-274

Schonthaler, H B; Lampert, J M; Isken, A; Rinner, O; Mader, A; Gesemann, M; Oberhauser, V; Golczak, M; Biehlmaier, O; Palczewski, K; Neuhauss, S C F; von Lintig, J (2007). Evidence for RPE65-independent vision in the cone-dominated zebrafish retina. European Journal of Neuroscience, 26(7):1940-1949.

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An enzyme-based cyclic pathway for trans to cis isomerization of the chromophore of visual pigments (11-cis-retinal) is intrinsic to vertebrate cone and rod vision. This process, called the visual cycle, is mostly characterized in rod-dominated retinas and essentially depends on RPE65, an all-trans to 11-cis-retinoid isomerase. Here we analysed the role of RPE65 in zebrafish, a species with a cone-dominated retina. We cloned zebrafish RPE65 and showed that its expression coincided with photoreceptor development. Targeted gene knockdown of RPE65 resulted in morphologically altered rod outer segments and overall reduced 11-cis-retinal levels. Cone vision of RPE65-deficient larvae remained functional as demonstrated by behavioural tests and by metabolite profiling for retinoids. Furthermore, all-trans retinylamine, a potent inhibitor of the rod visual cycle, reduced 11-cis-retinal levels of control larvae to a similar extent but showed no additive effects in RPE65-deficient larvae. Thus, our study of zebrafish provides in vivo evidence for the existence of an RPE65-independent pathway for the regeneration of 11-cis-retinal for cone vision.


31 citations in Web of Science®
31 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Date:26 October 2007
Deposited On:11 Feb 2008 12:14
Last Modified:05 Apr 2016 12:13
Additional Information:The definitive version is available at www.blackwell-synergy.com.
Publisher DOI:10.1111/j.1460-9568.2007.05801.x
PubMed ID:17868371

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