Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-27840
Cesarovic, N. Acute and long-term effects of isoflurane and sevoflurane anaesthesia in laboratory mice. 2009, University of Zurich, Vetsuisse Faculty.
Isoflurane is to date the most common volatile anaesthetic in laboratory rodents whereas the modern sevoflurane is usual for inhalation anaesthesia in human medicine. In this study it was aimed to characterize and compare the clinical properties and safety of both anaesthetics for anaesthetizing mice. In an approach that mirrors the laboratory routine (spontaneous breathing, gas supply via nose mask, preventing hypothermia by a warming mat) a 50-minutes anaesthesia was performed. Anaesthetics were administered at standardized dosages of 1.5 X minimum alveolar concentrations (1.85% for isoflurane, 3.25% for sevoflurane) in 100% oxygen. Induction and recovery from anaesthesia proceeded quickly, within 1 and 2 minutes respectively. During anaesthesia, all reflex testing were negative and no serious impairment of vital functions was found; all animals survived. Most prominent side effect during anaesthesia was the respiratory depression with marked decrease of respiration rate, hypercapnia and acidosis. Under anaesthesia, heart rate and core body temperature remained stable and within normal range, but were significantly increased for 12 hours after anaesthesia. Locomotor activity, food and water consumption and body weight progression showed no abnormalities after anaesthesia. No relevant differences between the two anaesthetics were found. In conclusion, both anaesthetics equally provide high safety margin with acceptable side effects and are therefore recommended for anaesthesia in laboratory mice.
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|Referees:||Bürki K, Lutz T|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Laboratory Animal Science|
|Dewey Decimal Classification:||570 Life sciences; biology
610 Medicine & health
|Deposited On:||20 Jan 2010 08:07|
|Last Modified:||09 Jul 2012 04:08|
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