Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-27856

Lee, S B; Schramme, A; Doberstein, K; Dummer, R; Abdel-Bakky, M S; Keller, S; Altevogt, P; Oh, S T; Reichrath, J; Oxmann, D; Pfeilschifter, J; Mihic-Probst, D; Gutwein, P (2010). ADAM10 is upregulated in melanoma metastasis compared with primary melanoma. Journal of Investigative Dermatology, 130(3):763-773.

[img] PDF - Registered users only
5MB

View at publisher

Abstract

ADAM10 (a disintegrin and metalloproteinase 10) is involved in the ectodomain shedding of various substrates, including adhesion molecules such as L1 cell adhesion molecule (L1-CAM) and CD44, which are known to have important roles in the development of malignant melanoma. In our study, we characterized the expression of ADAM10 in melanoma cells in vitro and in vivo. Immunohistochemical analysis on tissue microarrays indicated that ADAM10 expression was significantly elevated in melanoma metastasis compared with primary melanomas. In vitro downregulation of ADAM10 with specific small interfering RNA (siRNA) resulted in a suppression of the anchorage-independent cell growth and reduced the migration of melanoma cells. In addition, overexpression of ADAM10 induced the migration of melanoma cells. In cell lines from melanoma patients with metastasis, ADAM10 was significantly overexpressed, and ADAM10 expression correlated with increased cell proliferation. Furthermore, we present evidence that ADAM10 is involved in the release of L1-CAM from melanoma cells. It is important that knockdown of cellular L1-CAM reduced the migration of melanoma cells and abrogated the chemoresistance against cisplatin. In contrast, soluble L1-CAM had no effect on melanoma cell migration or cell survival. Taken together, our data demonstrate that ADAM10 and L1-CAM have important roles during melanoma progression and both molecules represent attractive targets for therapeutical intervention of melanomas.Journal of Investigative Dermatology advance online publication, 29 October 2009; doi:10.1038/jid.2009.335.

Citations

25 citations in Web of Science®
30 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

3 downloads since deposited on 30 Jan 2010
0 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
04 Faculty of Medicine > University Hospital Zurich > Institute of Surgical Pathology
DDC:610 Medicine & health
Language:English
Date:March 2010
Deposited On:30 Jan 2010 11:35
Last Modified:04 Dec 2013 16:44
Publisher:Nature Publishing Group
ISSN:0022-202X
Publisher DOI:10.1038/jid.2009.335
PubMed ID:19865098

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page