Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-28049
Furler, M; Knobloch, B; Sigel, R K O (2009). Influence of decreased solvent permittivity on the structure and magnesium(II)-binding properties of the catalytic domain 5 of a group II intron ribozyme. Inorganica Chimica Acta, 362(3):771-776.
View at publisher
Although it is well known that the so-called “equivalent solution” or “effective” solvent permittivity (dielectric constant) in proteins and nucleic acids is lower than in bulk water, this fact is commonly neglected in (bioinorganic) studies of such compounds. Using domain 5 of the group II intron ribozyme Sc.ai5γ, we describe here the influence of 1,4-dioxane-d8 on the structure and magnesium(II)-binding properties of this catalytic domain. Applying one- and two-dimensional NMR, we observe distinct structural changes in the functionally important bulge region following a decrease in solvent permittivity. Concomitantly, an increase by a factor of 1.5 in the affinity of Mg2+ towards the individual-binding sites in the catalytic core domain is observed upon addition of 1,4-dioxane-d8. This has led to the detection of a new metal ion coordination site near the GU wobble pair in the catalytic triad. Our results show that solvent permittivity is an important factor in the formation of intrinsic RNA structures and affects their metal ion-binding properties. Hence, solvent permittivity should be taken into account in future studies.
26 downloads since deposited on 22 Jan 2010
16 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Department of Chemistry|
|Deposited On:||22 Jan 2010 11:19|
|Last Modified:||29 Nov 2013 13:27|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page