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El-Badry, A M; Breitenstein, S; Jochum, W; Washington, K; Paradis, V; Rubbia-Brandt, L; Puhan, M A; Slankamenac, K; Graf, R; Clavien, P A (2009). Assessment of hepatic steatosis by expert pathologists: the end of a gold standard. Annals of Surgery, 250(5):691-697.

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Abstract

BACKGROUND: The presence of fat in the liver is considered a major risk for postoperative complication after liver surgery and transplantation. The current standard of quantification of hepatic steatosis is microscopic evaluation by pathologists, although consistency in such assessment remains unclear. Computerized image analysis is an alternative method for objective assessment of the degree of hepatic steatosis. METHODS: High resolution images of hematoxylin and eosin stained liver sections from 46 consecutive patients, initially diagnosed with liver steatosis, were blindly assessed by 4 established expert pathologists from different institutions. Computerized analysis was carried out simultaneously on the same sections. Interobserver agreement and correlation between the pathologists' and computerized assessment were evaluated using intraclass correlation coefficients (ICC), Spearman rank correlation coefficients, or descriptive statistics. RESULTS: Poor agreement among pathologists (ICC: 0.57) was found regarding the assessment of total steatosis, (ICC >0.7 indicates acceptable agreement). Pathologists' estimation of micro- and macrosteatosis disclosed also poor correlation (ICC: 0.22, 0.55, respectively). Inconsistent assessment of histological features of steatohepatitis (lobular inflammation, portal inflammation, hepatocyte ballooning, and Mallory hyaline) was documented. Poor conformity was also shown between the computerized quantification and ratings of 3 pathologists (Spearman rank correlation coefficients: 0.22, 0.82, 0.28, and 0.38). CONCLUSION: Quantification of hepatic steatosis in histological sections is strongly observer-dependent, not reproducible, and does not correlate with the computerized estimation. Current standards of assessment, previously published data and the clinical relevance of hepatic steatosis for liver surgery and transplantation must be challenged.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > The Horten-Center for Applied Research and Science
04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine
04 Faculty of Medicine > University Hospital Zurich > Clinic for Visceral and Transplantation Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
DDC:610 Medicine & health
Language:English
Date:2009
Deposited On:25 Jan 2010 14:15
Last Modified:27 Nov 2013 16:46
Publisher:Lippincott Wiliams & Wilkins
ISSN:0003-4932
Publisher DOI:10.1097/SLA.0b013e3181bcd6dd
PubMed ID:19806055
Citations:Web of Science®. Times Cited: 76
Google Scholar™
Scopus®. Citation Count: 78

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