Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-28427

Rabe, Michael; Verdes, Dorinel; Seeger, Stefan (2009). Surface-induced spreading phenomenon of protein clusters. Soft Matter, 5(5):1039-1047.

[img] PDF - Registered users only
3MB

Abstract

In this study we investigate the behavior of protein clusters on solid surfaces. It is shown that clusters
consisting of up to several hundreds of protein monomers can form in solution even at low monomer
concentrations and subsequently adsorb to the surface. Using FRET imaging, a significant increase in
the inter-protein distance of cluster proteins in time (e.g. a 46% increase after 7 hours on a hydrophobic
surface) was revealed indicating that protein clusters spread upon contact with the surface. The
spreading rate was strongly dependent on the surface chemistry: fast spreading on hydrophobic, slow
spreading on hydrophilic surfaces. Moreover, we found that protein clusters could be deposited on
a hydrophobic, OTS-coated surface even though it was already covered with a layer of pre-adsorbed
protein monomers. As a result of their high surface mobility these monomers receded from that area to
which the protein clusters spread. The molecular rearrangements taking place when a protein cluster
deposits onto a surface are discussed in the context of the experimental results.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
DDC:540 Chemistry
Language:English
Date:20 January 2009
Deposited On:31 Jan 2010 19:00
Last Modified:05 Jun 2014 13:50
Publisher:Royal Society of Chemistry
ISSN:1744-683X
Additional Information:Persons who receive the PDF must not make it further available or distribute it
Publisher DOI:10.1039/b814053g
Citations:Web of Science®. Times Cited: 15
Google Scholar™
Scopus®. Citation Count: 17

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page