Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-28643

Kessler, R; Hausmann, G; Basler, K (2009). The PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3. Mechanisms of Development, 126(8-9):752-759.

[img] PDF - Registered users only
2MB

View at publisher

Abstract

In Drosophila Pygopus (Pygo) and Legless (Lgs)/BCL9 are integral components of the nuclear Wnt/Wg signaling machine. Despite intense research, ideas that account for their mode of action remain speculative. One proposition, based on a recently discovered function of PHD fingers, is that Pygo, through its PHD, may decipher the histone code. We found that human, but not Drosophila, Pygo robustly interacts with a histone-H3 peptide methylated at lysine-4. The different binding behavior is due to a single amino acid change that appears unique to Drosophilidae Pygo proteins. Rescue experiments with predicted histone binding mutants showed that in Drosophila the ability to bind histones is not essential. Further experiments with Pygo-Lgs fusions instead demonstrated that the crucial role of the PHD is to provide an interaction motif to bind Lgs. Our results reveal an interesting evolutionary dichotomy in Pygo structure-function, as well as evidence underpinning the chain of adaptors model.

Citations

10 citations in Web of Science®
10 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 29 Jan 2010
0 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:2009
Deposited On:29 Jan 2010 12:47
Last Modified:27 Nov 2013 19:31
Publisher:Elsevier
ISSN:0925-4773
Publisher DOI:10.1016/j.mod.2009.04.003
PubMed ID:19493659

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page