Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-2867
Wenger, D; Gerecke, A; Heeb, N; Naegeli, H; Zenobi, R (2008). Catalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust. Analytical and Bioanalytical Chemistry, 390(8):2021-2029.
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An in vitro reporter gene assay based on human breast cancer T47D cells (ER-CALUX) was applied to examine the ability of diesel exhaust to induce or inhibit estrogen receptor (ER)-mediated gene expression. Exhaust from a heavy-duty diesel engine was either treated by iron- or copper/iron-catalyzed diesel particulate filters (DPFs) or studied as unfiltered exhaust. Collected samples included particle-bound and semivolatile constituents of diesel exhaust. Our findings show that all of the samples contained compounds that were able to induce ER-mediated gene expression as well as compounds that suppressed the activity of the endogenous hormone 17beta-estradiol (E2). Estrogenic activity prevailed over antiestrogenic activity. We found an overall ER-mediated activity of 1.63 +/- 0.31 ng E2 CALUX equivalents (E2-CEQs) per m(3) of unfiltered exhaust. In filtered exhaust, we measured 0.74 +/- 0.07 (iron-catalyzed DPF) and 0.55 +/- 0.09 ng E2-CEQ m(-3) (copper/iron-catalyzed DPF), corresponding to reductions in estrogenic activity of 55 and 66%, respectively. Our study demonstrates that both catalytic DPFs lowered the ER-mediated endocrine-disrupting potential of diesel exhaust.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Veterinary Pharmacology and Toxicology|
|Dewey Decimal Classification:||570 Life sciences; biology|
|Deposited On:||25 Aug 2008 07:09|
|Last Modified:||05 Apr 2016 12:24|
|Funders:||Swiss National Science Foundation|
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