Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-28741
Spirov, A; Fahmy, K; Schneider, M; Frei, E; Noll, M; Baumgartner, S (2009). Formation of the bicoid morphogen gradient: an mRNA gradient dictates the protein gradient. Development, 136(4):605-614.
View at publisher
The Bicoid (Bcd) protein gradient is generally believed to be established in pre-blastoderm Drosophila embryos by the diffusion of Bcd protein after translation of maternal mRNA, which serves as a strictly localized source of Bcd at the anterior pole. However, we previously published evidence that the Bcd gradient is preceded by a bcd mRNA gradient. Here, we have revisited and extended this observation by showing that the bcd mRNA and Bcd protein gradient profiles are virtually identical at all times. This confirms our previous conclusion that the Bcd gradient is produced by a bcd mRNA gradient rather than by diffusion. Based on our observation that bcd mRNA colocalizes with Staufen (Stau), we propose that the bcd mRNA gradient forms by a novel mechanism involving quasi-random active transport of a Stau-bcd mRNA complex through a nonpolar microtubular network, which confines the bcd mRNA to the cortex of the embryo.
55 downloads since deposited on 05 Feb 2010
16 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|Dewey Decimal Classification:||570 Life sciences; biology|
|Deposited On:||05 Feb 2010 10:19|
|Last Modified:||27 Nov 2013 23:08|
|Publisher:||Company of Biologists|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page