Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Rittirsch, D; Huber-Lang, M S; Flierl, M A; Ward, P A (2009). Immunodesign of experimental sepsis by cecal ligation and puncture. Nature Protocols, 4(1):31-36.

Full text not available from this repository.

Abstract

Sepsis remains a prevalent clinical challenge and the underlying pathophysiology is still poorly understood. To investigate the complex molecular mechanisms of sepsis, various animal models have been developed, the most frequently used being the cecal ligation and puncture (CLP) model in rodents. In this model, sepsis originates from a polymicrobial infectious focus within the abdominal cavity, followed by bacterial translocation into the blood compartment, which then triggers a systemic inflammatory response. A requirement of this model is that it is performed with high consistency to obtain reproducible results. Evidence is now emerging that the accompanying inflammatory response varies with the severity grade of sepsis, which is highly dependent on the extent of cecal ligation. In this protocol, we define standardized procedures for inducing sepsis in mice and rats by applying defined severity grades of sepsis through modulation of the position of cecal ligation. The CLP procedure can be performed in as little as 10 min for each animal by an experienced user, with additional time required for subsequent postoperative care and data collection.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Trauma Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
DDC:610 Medicine & health
Language:English
Date:2009
Deposited On:01 Feb 2010 16:24
Last Modified:27 Nov 2013 18:59
Publisher:Nature Publishing Group
ISSN:1750-2799
Publisher DOI:10.1038/nprot.2008.214
PubMed ID:19131954
Citations:Web of Science®. Times Cited: 187
Google Scholar™
Scopus®. Citation Count: 216

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page