Onai, N; Manz, M G; Schmid , M A (2010). Isolation of common dendritic cell progenitors (CDP) from mouse bone marrow. In: Naik, S H. Dendritic Cell Protocols. United States, 195-203. ISBN 978-1-60761-420-3 (P) 978-1-60761-421-0 (E).
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In the steady-state lymphoid organ, dendritic cells (DCs) are classified into two major subsets, plasmacytoid DC (pDC) and conventional DC (cDC). A standing question was whether a common progenitor for plasmacytoid and conventional dendritic cells exists during the sequential differentiation from hematopoietic stem cells to dendritic cells. We have recently identified such a common clonogenic plasmacytoid and dendritic cell progenitor (CDP) from mouse bone marrow using antibodies for c-kit, Flt3, and M-CSFR. CDPs generated almost exclusively pDC and cDC in vitro and upon transfer in irradiated and steady-state mice in vivo. Single-cell analysis revealed the existence of clonal progenitors giving rise to both pDC and cDC within the CDP population. Thus, these results prove the existence of a common developmental pathway for at least some pDCs and cDCs in lymphoid organs in vivo.
|Item Type:||Book Section, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Hematology|
04 Faculty of Medicine > University Hospital Zurich > Clinic for Immunology
|DDC:||610 Medicine & health|
|Deposited On:||02 Mar 2010 15:15|
|Last Modified:||27 Nov 2013 18:26|
|Publisher:||Springer / Humana Press|
|Series Name:||Methods in Molecular Biology|
|Number:||Volume 595 / Part 3|
|ISSN:||1064-3745 (P) 1940-6029 (E)|
|ISBN:||978-1-60761-420-3 (P) 978-1-60761-421-0 (E)|
|Citations:||Web of Science®. Times cited: 3|
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