Wojas, S; Hennig, J; Plaza, S; Geisler, M; Siemianowski, O; Sklodowska, A; Ruszczynska, A; Bulska, E; Antosiewicz, D M (2009). Ectopic expression of Arabidopsis ABC transporter MRP7 modifies cadmium root-to-shoot transport and accumulation. Environmental Pollution, 157(10):2781 - 2789.
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Arabidopsis MRPs/ABCCs have been shown to remove various organic and inorganic substrates from the cytosol to other subcellular compartments. Here we first demonstrate that heterologous expression of AtMRP7 in tobacco (Nicotiana tabacum var. Xanthi) modifies cadmium accumulation, distribution and tolerance. Arabidopsis MRP7 was localized both in the tonoplast and in the plasma membrane when expressed in tobacco. Its overexpression increased tobacco Cd-tolerance and resulted in enhanced cadmium concentration in leaf vacuoles, indicating more efficient detoxification by means of vacuolar storage. Heterologous AtMRP7 expression also led to more efficient retention of Cd in roots, suggesting a contribution to the control of cadmium root-to-shoot translocation. The results underscore the use of AtMRP7 in plant genetic engineering to modify the heavy-metal accumulation pattern for a broad range of applications.
AtMRP7 expression in tobacco enhances Cd-tolerance and increases Cd storage in vacuoles
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Plant Biology|
|Dewey Decimal Classification:||580 Plants (Botany)|
|Uncontrolled Keywords:||Plants, tobacco|
|Deposited On:||04 Feb 2010 18:37|
|Last Modified:||27 Nov 2013 22:10|
|Free access at:||Related URL. An embargo period may apply.|
|Related URLs:||http://botserv1.uzh.ch/home/martinoia/pdf/Wojas%20et%20al.%20(2009).pdf (Organisation)|
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