Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Rühli, Frank J; Kuhn, G; Evison, R; Müller, R; Schultz, M (2007). Diagnostic value of micro-CT in comparison with histology in the qualitative assessment of historical human skull bone pathologies. American Journal of Physical Anthropology, 133(4):1099-1111.

Full text not available from this repository.

View at publisher

Abstract

Cases of pathologically changed bone might constitute a diagnostic pitfall and frequently need histological methods to be etiologically properly evaluated. With micro-computed tomography (microCT), a new epoch of 2D and 3D imaging has been launched. We evaluated the diagnostic investigation of this analytical method versus well established histological investigations of historical human bone. Pathological changes due to various etiologies (infectious, traumatic, endocrinological, neoplasia) observed in autopsy-based macerated human skulls (Galler Collection, Natural History Museum Basel, Switzerland) were investigated by microCT and compared with histological thin ground sections using polarized light. Micro-CT images visualize the architecture of the bone with high spatial resolution without preparation or destruction of the sample in the area to be sectioned. Changes in the bone surfaces as well as alterations of the diploë can be assessed. However, morphological patterns caused by reactive response, such as typical arrangements of collagen fibers, can only be visualized by the microscopic investigation of thin ground sections using polarized light. A great advantage of microCT is the high number of slices obtained so that spatial differences within the areas of the specimen become visible. Micro-CT is a valuable tool for the diagnosis of vestiges of skull bone diseases. Its advantages over histology are the fast, automated image acquisition and the fact that the specimen is not completely destroyed. Only excision of the area to be scanned is necessary, if the specimen is too large to be scanned as a whole. Further, the 3D visualization of the micro-architecture allows an easy orientation within the sample, for example, for the choice of the location of the histological slices. However, the need to differentiate woven from lamellar bone still makes histology an indispensable method.

Citations

13 citations in Web of Science®
17 citations in Scopus®
Google Scholar™

Altmetrics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Evolutionary Medicine
04 Faculty of Medicine > Institute of Anatomy
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:August 2007
Deposited On:24 Mar 2010 15:17
Last Modified:25 Sep 2014 11:32
Publisher:Wiley-Blackwell
ISSN:0002-9483
Publisher DOI:10.1002/ajpa.20611
PubMed ID:17530700

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page