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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-29972

Eppler, E; Hörig, H; Kaufman, H L; Groscurth, P; Filgueira, L (2002). Carcinoembryonic antigen (CEA) presentation and specific T cell-priming by human dendritic cells transfected with CEA-mRNA. European Journal of Cancer, 38(1):184-193.

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Abstract

The feasibility of dendritic cells (DC) for cancer immunotherapy after transfection by electroporation with mRNA encoding the human carcinoembryonic antigen (CEA) was investigated. Both, total RNA from the CEA(+) colon cancer cell line SW480 and mRNA transcribed in vitro from cDNA3.1-plasmids (pcDNA3.1+/-HisC) with a CEA-insert (ivt-CEA-mRNA, ivt-CEA/HisC-mRNA) were used. Labelled ivt-CEA-mRNA was detectable in DC by light and electron microscopy and by fluorescence-activated cell-sorting (FACS) even 15 min after electroporation. Four hours after transfection with ivt-CEA/HisC-mRNA, we detected specific expression of CEA and the histidine-tag by immunofluorescence microscopy and by FACS. CEA-specific T lymphocytes were successfully primed by transfected DC and were able to lyse CEA-expressing target cells, even from the CEA-expressing human colon adenocarcinoma cell line SW480. Thus, DC transfected by electroporation with CEA-mRNA are valuable tools for the immunotherapy of CEA(+) tumour entities.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Anatomy
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:January 2002
Deposited On:26 Mar 2010 08:39
Last Modified:23 Nov 2012 14:19
Publisher:Elsevier
ISSN:0959-8049
Publisher DOI:10.1016/S0959-8049(01)00342-2
PubMed ID:11750849

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