Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Reichel, C; Gao, B; Van Montfoort, J; Cattori, V; Rahner, C; Hagenbuch, B; Stieger, B; Kamisako, T; Meier, P J (1999). Localization and function of the organic anion-transporting polypeptide Oatp2 in rat liver. Gastroenterology, 117(3):688-695.

Full text not available from this repository.

View at publisher


BACKGROUND & AIMS: Multispecific organic anion-transporting polypeptides (Oatps) are involved in the transcellular movement of amphipathic compounds in many tissues including the liver, kidney, and blood-brain barrier. Recently, a high-affinity digoxin transporter (Oatp2) was cloned from rat brain and shown to be also expressed in the liver. METHODS: We investigated the cellular and subcellular distribution of Oatp2 in rat liver by in situ hybridization technology and immunofluorescence microscopy and compared its substrate specificity with that of Oatp1 in complementary RNA-injected Xenopus laevis oocytes. RESULTS: The results show a selective basolateral (sinusoidal) expression of Oatp2 in midzonal to perivenous hepatocytes, but not in periportal or the innermost layer of perivenous hepatocytes. Common substrates of both Oatp1 and Oatp2 include bile salts, steroid conjugates, thyroid hormones (T3, T4), ouabain, and the endothelin receptor antagonist BQ-123 (Michaelis constants: Oatp1, approximately 600 micromol/L; Oatp2, approximately 30 micromol/L). Other organic anions including sulfolithotaurocholate, bilirubin monoglucuronide, and sulfobromophthalein were transported only by Oatp1. CONCLUSIONS: These results provide definite evidence for the partially overlapping and partially selective substrate specificities of Oatp1 and Oatp2. The unique acinar distribution of Oatp2 might indicate that it represents a high-affinity "backup" system for complete hepatocellular removal of certain cholephilic substances from portal blood plasma.


177 citations in Web of Science®
180 citations in Scopus®
Google Scholar™


Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Clinical Pharmacology and Toxicology
Dewey Decimal Classification:610 Medicine & health
Deposited On:07 Apr 2009 15:07
Last Modified:05 Apr 2016 12:25
Additional Information:Elsevier - Full-text article
Publisher DOI:10.1016/S0016-5085(99)70463-4
Official URL:http://linkinghub.elsevier.com/retrieve/pii/S001650859900268
PubMed ID:10464146

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page