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Molecular cloning and functional characterization of two alternatively spliced Ntcp isoforms from mouse liver1


Cattori, V; Eckhardt, U; Hagenbuch, B (1999). Molecular cloning and functional characterization of two alternatively spliced Ntcp isoforms from mouse liver1. Biochimica et Biophysica Acta, 1445(1):154-159.

Abstract

To isolate the murine Na+/taurocholate cotransporting polypeptide (Ntcp), we screened a mouse liver cDNA library and identified Ntcp1, encoding a 362 amino acid protein and Ntcp2, encoding a 317 amino acid protein which had a shorter C-terminal end. Both isoforms mediated saturable Na+-dependent transport of taurocholate when expressed in Xenopus laevis oocytes. Analysis of the gene revealed that Ntcp2 is produced by alternative splicing where the last intron is retained.

To isolate the murine Na+/taurocholate cotransporting polypeptide (Ntcp), we screened a mouse liver cDNA library and identified Ntcp1, encoding a 362 amino acid protein and Ntcp2, encoding a 317 amino acid protein which had a shorter C-terminal end. Both isoforms mediated saturable Na+-dependent transport of taurocholate when expressed in Xenopus laevis oocytes. Analysis of the gene revealed that Ntcp2 is produced by alternative splicing where the last intron is retained.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Clinical Pharmacology and Toxicology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:14 April 1999
Deposited On:08 Apr 2009 09:32
Last Modified:05 Apr 2016 12:25
Publisher:Elsevier
ISSN:0006-3002
Publisher DOI:https://doi.org/10.1016/S0167-4781(99)00029-9
PubMed ID:10209268

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