Düber, S; Hafner, M; Krey, M; Lienenklaus, S; Roy, B; Hobeika, E; Reth, M; Buch, T; Waisman, A; Kretschmer, K; Weiss, S (2009). Induction of B-cell development in adult mice reveals the ability of bone marrow to produce B-1a cells. Blood, 114(24):4960-4967.
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To study B-cell development from bone marrow (BM), we generated recombination-activating gene 1 (Rag1)-targeted mice lacking mature lymphocytes. B-cell development can be induced in such mice by B cell-specific restoration of a functional Rag1 transcription unit. Follicular and marginal zone B cells populated the spleen when Rag1 expression was permitted. Notably, the peritoneal cavity was dominated by bona fide B-1a cells, as judged by surface markers and functional properties. These BM-derived B-1a cells exhibited a polyclonal VDJ repertoire with substantial N nucleotide insertions. Nevertheless, physiologic frequencies of phosphatidylcholine-specific B cells were detected. Importantly, the BM of young and 5-month-old mice was indistinguishable with regard to the potential to generate B-1a cells.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Experimental Immunology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||11 Feb 2010 20:20|
|Last Modified:||23 Nov 2012 16:23|
|Publisher:||American Society of Hematology|
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