Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-3024
Leeming, G; Meli, M L; Cripps, P; Vaughan-Thomas, A; Lutz, H; Gaskell, R; Kipar, A (2006). Tracheal organ cultures as a useful tool to study Felid herpesvirus 1 infection in respiratory epithelium. Journal of Virological Methods, 138(1-2):191-195.
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Felid herpesvirus 1 (FeHV-1) is an important feline pathogen of the upper respiratory tract which can be identified in clinical cases by virus isolation and PCR. Studies on the effect and mode of spread of FeHV-1 in the respiratory epithelium, however, have previously only been performed in infected cats. Feline tracheal organ cultures which were inoculated with FeHV-1 at varying multiplicity of infection (MOI) were established. A dose-dependent response was observed. Low MOIs induced multifocal infection in the otherwise viable respiratory epithelium, which allowed monitoring of viral growth over several days. Therefore, tracheal organ cultures represent a suitable model for further study of the morphological and functional effects of FeHV-1 on respiratory epithelium, mimicking the in vivo situation.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals > Clinical Laboratory|
|DDC:||570 Life sciences; biology|
|Deposited On:||18 Aug 2008 10:14|
|Last Modified:||27 Nov 2013 17:28|
|Citations:||Web of Science®. Times cited: 4|
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