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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-30403

Altintas, M A; Altintas, A A; Guggenheim, M; Steiert, A E; Aust, M C; Niederbichler, A D; Herold, C; Vogt, P M (2010). Insight in human skin microcirculation using in vivo reflectance-mode confocal laser scanning microscopy. Journal of Digital Imaging, 23(4):475-481.

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Abstract

Reflectance-mode confocal laser scanning microscopy allows in vivo imaging of the human skin. We hypothesized that this high-resolution technique enables observation of dynamic changes of the cutaneous microcirculation. Twenty-two volunteers were randomly divided in two groups. Group 1 was exposed to local heating and group 2 to local cold stress. Confocal microscopy was performed prior t (0) (control), directly t (1) and 5 min t (2) after local temperature changes to evaluate quantitative blood cell flow, capillary loop diameter, and density of dermal capillaries. In group 1, blood flow increased at t (1) (75.82 +/- 2.86/min) and further at t (2) (84.09 +/- 3.39/min) compared to the control (61.09 +/- 3.21/min). The control capillary size was 9.59 +/- 0.25 mum, increased to 11.16 +/- 0.21 mum (t (1)) and 11.57 +/- 0.24 mum (t (2)). The dermal capillary density increased in t (1) (7.26 +/- 0.76/mm(2)) and t (2) (8.16 +/- 0.52/mm(2)), compared to the control (7.04 +/- 0.62/mm(2)). In group 2, blood flow decreased at t (1) (41.73 +/- 2.61/min) and increased at t (2) (83.27 +/- 3.29/min) compared to the control (60.73 +/- 2.90/min). The control capillary size was 9.55 +/- 0.25 mum, decreased at t (1) (7.78 +/- 0.26 mum) and increased at t (2) (11.38 +/- 0.26 mum). Capillary density decreased at t (1) (5.01 +/- 0.49/mm(2)) and increased at t (2) (7.28 +/- 0.53/mm(2)) compared to the control (7.01 +/- 0.52/mm(2)). Confocal microscopy is a sensitive and noninvasive imaging tool for characterizing and quantifying dynamic changes of cutaneous microcirculation on a histomorphological level.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
DDC:610 Medicine & health
Language:English
Date:1 August 2010
Deposited On:11 Feb 2010 17:27
Last Modified:05 Dec 2013 06:55
Publisher:Springer
ISSN:0897-1889
Publisher DOI:10.1007/s10278-009-9219-3
PubMed ID:19513794
Citations:Web of Science®. Times Cited: 5
Google Scholar™
Scopus®. Citation Count: 6

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