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Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat


Johnson, P C D; Webster, L M I; Adam, A; Buckland, R; Dawson, D A; Keller, L F (2006). Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. Molecular and Biochemical Parasitology, 148(2):210-218.

Abstract

An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average H-e = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F-ST = 0.00) or within hosts (F-IS = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus.

An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average H-e = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F-ST = 0.00) or within hosts (F-IS = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:2006
Deposited On:27 Mar 2009 13:39
Last Modified:05 Apr 2016 12:25
Publisher:Elsevier
ISSN:0166-6851
Publisher DOI:10.1016/j.molbiopara.2006.04.011
Permanent URL: http://doi.org/10.5167/uzh-3103

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