Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-31257

Godthelp, B C; Wiegant, W W; van Duijn-Goedhart, A; Schärer, O D; van Buul, P P W; Kanaar, R; Zdzienicka, M Z (2002). Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Nucleic Acids Research, 30(10):2172-2182.

[img]
Preview
PDF
696kB

View at publisher

Abstract

The eukaryotic Rad51 protein is a structural and functional homolog of Escherichia coli RecA with a role in DNA repair and genetic recombination. Five paralogs of Rad51 have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, which are also implicated in recombination and genome stability. Here, we identify a mammalian cell mutant in Rad51C. We show that the Chinese hamster cell mutant, CL-V4B, has a defect in Rad51C. Sequencing of the hamster Rad51C cDNA revealed a 132 bp deletion corresponding to an alternatively spliced transcript with lack of exon 5. CL-V4B was hypersensitive to the interstrand cross-linking agents mitomycin C (MMC) and cisplatinum, the alkylating agent methyl methanesulfonate and the topoisomerase I inhibitor campthotecin and showed impaired Rad51 foci formation in response to DNA damage. The defect in Rad51C also resulted in an increase of spontaneous and MMC-induced chromosomal aberrations as well as a lack of induction of sister chromatid exchanges. However, centrosome formation was not affected. Intriguingly, a reduced level of sister chromatid cohesion was found in CL-V4B cells. These results reveal a role for Rad51C that is unique among the Rad51 paralogs.

Citations

106 citations in Web of Science®
107 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

19 downloads since deposited on 09 Jul 2010
4 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Molecular Cancer Research
07 Faculty of Science > Institute of Molecular Cancer Research
DDC:570 Life sciences; biology
Language:English
Date:2002
Deposited On:09 Jul 2010 09:10
Last Modified:01 Jan 2014 19:44
Publisher:Oxford University Press
ISSN:0305-1048
Publisher DOI:10.1093/nar/30.10.2172
Official URL:http://nar.oxfordjournals.org/cgi/content/full/30/10/2172
PubMed ID:12000837

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page