Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-31398

Nair, S K; Wang, N; Turuspekov, Y; Pourkheirandish, M; Sinsuwongwat, S; Chen, G; Sameri, M; Tagiri, A; Honda, I; Watanabe, Y; Kanamori, H; Wicker, T; Stein, N; Nagamura, Y; Matsumoto, T; Komatsuda, T (2010). Cleistogamous flowering in barley arises from the suppression of microRNA-guided HvAP2 mRNA cleavage. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107(1):490-495.

[img]
Preview
PDF
2178Kb

Abstract

The cleistogamous flower sheds its pollen before opening, forcing plants with this habit to be almost entirely autogamous. Cleistogamy also provides a means of escape from cereal head blight infection and minimizes pollen-mediated gene flow. The lodicule in cleistogamous barley is atrophied. We have isolated cleistogamy 1 (Cly1) by positional cloning and show that it encodes a transcription factor containing two AP2 domains and a putative microRNA miR172 targeting site, which is an ortholog of Arabidopsis thaliana AP2. The expression of Cly1 was concentrated within the lodicule primordia. We established a perfect association between a synonymous nucleotide substitution at the miR172 targeting site and cleistogamy. Cleavage of mRNA directed by miR172 was detectable only in a noncleistogamous background. We conclude that the miR172-derived down-regulation of Cly1 promotes the development of the lodicules, thereby ensuring noncleistogamy, although the single nucleotide change at the miR172 targeting site results in the failure of the lodicules to develop properly, producing the cleistogamous phenotype.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Plant Biology
DDC:580 Plants (Botany)
Language:English
Date:2010
Deposited On:18 Feb 2010 21:00
Last Modified:27 Nov 2013 19:07
Publisher:National Academy of Sciences
ISSN:0027-8424
Additional Information:Copyright: National Academy of Sciences USA
Publisher DOI:10.1073/pnas.0909097107
PubMed ID:20018663
Citations:Web of Science®. Times Cited: 31
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page