Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-31474
Hasan, S; El-Andaloussi, N; Hardeland, U; Hassa, P O; Bürki, C; Imhof, R; Schär, P; Hottiger, M O (2002). Acetylation regulates the DNA end-trimming activity of DNA polymerase beta. Molecular Cell, 10(5):1213-1222.
- Registered users only
View at publisher
We describe a novel regulatory mechanism for DNA polymerase beta (Polbeta), a protein involved in DNA base excision repair (BER). Polbeta colocalized in vivo and formed a complex with the transcriptional coactivator p300. p300 interacted with Polbeta through distinct domains and acetylated Polbeta in vitro. Polbeta acetylation was furthermore observed in vivo. Lysine 72 of Polbeta was identified as the main target for acetylation by p300. Interestingly, acetylated Polbeta showed a severely reduced ability to participate in a reconstituted BER assay. This was due to an impairment of the dRP-lyase activity of Polbeta. Acetylation of Polbeta thus acts as an intranuclear regulatory mechanism and implies that p300 plays a critical regulatory role in BER.
1 download since deposited on 09 Jun 2010
0 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Molecular Cancer Research
07 Faculty of Science > Institute of Molecular Cancer Research
|Dewey Decimal Classification:||570 Life sciences; biology|
|Deposited On:||09 Jun 2010 09:22|
|Last Modified:||05 Apr 2016 13:58|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page