Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-31474
Hasan, S; El-Andaloussi, N; Hardeland, U; Hassa, P O; Bürki, C; Imhof, R; Schär, P; Hottiger, M O (2002). Acetylation regulates the DNA end-trimming activity of DNA polymerase beta. Molecular Cell, 10(5):1213-1222.
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We describe a novel regulatory mechanism for DNA polymerase beta (Polbeta), a protein involved in DNA base excision repair (BER). Polbeta colocalized in vivo and formed a complex with the transcriptional coactivator p300. p300 interacted with Polbeta through distinct domains and acetylated Polbeta in vitro. Polbeta acetylation was furthermore observed in vivo. Lysine 72 of Polbeta was identified as the main target for acetylation by p300. Interestingly, acetylated Polbeta showed a severely reduced ability to participate in a reconstituted BER assay. This was due to an impairment of the dRP-lyase activity of Polbeta. Acetylation of Polbeta thus acts as an intranuclear regulatory mechanism and implies that p300 plays a critical regulatory role in BER.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Molecular Cancer Research|
07 Faculty of Science > Institute of Molecular Cancer Research
|DDC:||570 Life sciences; biology|
|Deposited On:||09 Jun 2010 11:22|
|Last Modified:||08 Dec 2012 12:59|
|WoS Citation Count:||59|
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