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Drosophila brain development: closing the gap between a macroarchitectural and microarchitectural approach


Cardona, A; Saalfeld, S; Tomancak, P; Hartenstein, V (2009). Drosophila brain development: closing the gap between a macroarchitectural and microarchitectural approach. In: Stillman, B; Stewart, D; Witkowski, J. Evolution: The molecular landscape. Cold Spring Harbor, NY, US: Cold Spring Harbor Laboratory Press, 235-248.

Abstract

Neurobiologists address neural structure, development, and function at the level of "macrocircuits" (how different brain compartments are interconnected; what overall pattern of activity they produce) and at the level of "microcircuits" (how connectivity and physiology of individual neurons and their processes within a compartment determine the functional output of this compartment). Work in our lab aims at reconstructing the developing Drosophila brain at both levels. Macrocircuits can be approached conveniently by reconstructing the pattern of brain lineages, which form groups of neurons whose projections form cohesive fascicles interconnecting the compartments of the larval and adult brain. The reconstruction of microcircuits requires serial section electron microscopy, due to the small size of terminal neuronal processes and their synaptic contacts. Because of the amount of labor that traditionally comes with this approach, very little is known about microcircuitry in brains across the animal kingdom. Many of the problems of serial electron microscopy reconstruction are now solvable with digital image recording and specialized software for both image acquisition and postprocessing. In this chapter, we introduce our efforts to reconstruct the small Drosophila larval brain and discuss our results in light of the published data on neuropile ultrastructure in other animal taxa.

Abstract

Neurobiologists address neural structure, development, and function at the level of "macrocircuits" (how different brain compartments are interconnected; what overall pattern of activity they produce) and at the level of "microcircuits" (how connectivity and physiology of individual neurons and their processes within a compartment determine the functional output of this compartment). Work in our lab aims at reconstructing the developing Drosophila brain at both levels. Macrocircuits can be approached conveniently by reconstructing the pattern of brain lineages, which form groups of neurons whose projections form cohesive fascicles interconnecting the compartments of the larval and adult brain. The reconstruction of microcircuits requires serial section electron microscopy, due to the small size of terminal neuronal processes and their synaptic contacts. Because of the amount of labor that traditionally comes with this approach, very little is known about microcircuitry in brains across the animal kingdom. Many of the problems of serial electron microscopy reconstruction are now solvable with digital image recording and specialized software for both image acquisition and postprocessing. In this chapter, we introduce our efforts to reconstruct the small Drosophila larval brain and discuss our results in light of the published data on neuropile ultrastructure in other animal taxa.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Neuroinformatics
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2009
Deposited On:01 Mar 2010 09:35
Last Modified:05 Apr 2016 13:59
Publisher:Cold Spring Harbor Laboratory Press
Series Name:Cold Spring Harbor Symposia on Quantitative Biology
Number:74
ISSN:0091-7451 (P) 1943-4456 (E)
ISBN:978-0-8796-9871-3
Additional Information:Evolution: The Molecular Landscape - 74th Cold Spring Harbor Symposium on Quantitative Biology, May 27 - June 1, 2009
Publisher DOI:https://doi.org/10.1101/sqb.2009.74.037
Related URLs:http://www.ini.uzh.ch/node/24110 (Organisation)
http://www.cshlpress.com/default.tpl?cart=126743543956939744&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=844 (Publisher)
http://symposium.cshlp.org/content/early/2009/12/18/sqb.2009.74.037.abstract
PubMed ID:20028843

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