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Prion protein library of recombinant constructs for structural biology


Hornemann, S; Christen, B; von Schroetter, C; Pérez, D R; Wüthrich, K (2009). Prion protein library of recombinant constructs for structural biology. FEBS Journal, 276(8):2359-2367.

Abstract

A survey of plasmids for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof, is presented. This includes information on the biochemistry of the recombinant proteins, in particular on successful and unsuccessful expression attempts. The plasmid library was generated during the past 12 years in the context of NMR structure determination and biophysical characterization of prion proteins in our laboratory. The plasmids are now available for general use, and are distributed free of charge to not-for-profit institutions.

A survey of plasmids for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof, is presented. This includes information on the biochemistry of the recombinant proteins, in particular on successful and unsuccessful expression attempts. The plasmid library was generated during the past 12 years in the context of NMR structure determination and biophysical characterization of prion proteins in our laboratory. The plasmids are now available for general use, and are distributed free of charge to not-for-profit institutions.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:03 Mar 2010 17:27
Last Modified:05 Apr 2016 14:00
Publisher:Wiley-Blackwell
ISSN:1742-464X
Publisher DOI:10.1111/j.1742-4658.2009.06968.x
PubMed ID:19348007
Permanent URL: http://doi.org/10.5167/uzh-32204

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