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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-3234

Springer, B; Sander, P; Sedlacek, L; Hardt, W D; Mizrahi, V; Schärer, P; Böttger, E C (2004). Lack of mismatch correction facilitates genome evolution in mycobacteria. Molecular Microbiology, 53(6):1601-1609.

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In silico genome sequence analyses suggested that mycobacteria are devoid of the highly conserved mutLS-based post-replicative mismatch repair system. Here, we present the first biological evidence for the lack of a classical mismatch repair function in mycobacteria. We found that frameshifts, but not general mutation rates are unusually high in Mycobacterium smegmatis. However, despite the absence of mismatch correction, M. smegmatis establishes a strong barrier to recombination between homeologous DNA sequences. We show that 10-12% of DNA sequence heterology restricts initiation of recombination but not extension of heteroduplex DNA intermediates. Together, the lack of mismatch correction and a high stringency of initiation of homologous recombination provide an adequate strategy for mycobacterial genome evolution, which occurs by gene duplication and divergent evolution.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
DDC:570 Life sciences; biology
610 Medicine & health
Date:August 2004
Deposited On:22 Aug 2008 12:31
Last Modified:27 Nov 2013 22:00
Publisher DOI:10.1111/j.1365-2958.2004.04231.x
PubMed ID:15341642
Citations:Web of Science®. Times Cited: 41
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Scopus®. Citation Count: 41

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