Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-3234
Springer, B; Sander, P; Sedlacek, L; Hardt, W D; Mizrahi, V; Schärer, P; Böttger, E C (2004). Lack of mismatch correction facilitates genome evolution in mycobacteria. Molecular Microbiology, 53(6):1601-1609.
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In silico genome sequence analyses suggested that mycobacteria are devoid of the highly conserved mutLS-based post-replicative mismatch repair system. Here, we present the first biological evidence for the lack of a classical mismatch repair function in mycobacteria. We found that frameshifts, but not general mutation rates are unusually high in Mycobacterium smegmatis. However, despite the absence of mismatch correction, M. smegmatis establishes a strong barrier to recombination between homeologous DNA sequences. We show that 10-12% of DNA sequence heterology restricts initiation of recombination but not extension of heteroduplex DNA intermediates. Together, the lack of mismatch correction and a high stringency of initiation of homologous recombination provide an adequate strategy for mycobacterial genome evolution, which occurs by gene duplication and divergent evolution.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Medical Microbiology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||22 Aug 2008 14:31|
|Last Modified:||23 Nov 2012 14:57|
|WoS Citation Count:||30|
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