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Influence of seminal plasma on fertility of fresh and frozen-thawed stallion epididymal spermatozoa


Heise, A; Kähn, W; Volkmann, D H; Thompson, P N; Gerber, D (2010). Influence of seminal plasma on fertility of fresh and frozen-thawed stallion epididymal spermatozoa. Animal Reproduction Science, 118(1):48-53.

Abstract

The use of epididymal stallion spermatozoa for routine artificial insemination can secure easy future use of valuable genetics after unforeseen death or injury of a valuable stallion. The aims of this study were to (1) directly compare pregnancy rates for fresh and frozen-thawed stallion epididymal and ejaculated spermatozoa after conventional artificial insemination and (2) to investigate the effect of seminal plasma on the fertility of epididymal spermatozoa after insemination. Twenty-one mares were randomly assigned to three stallions. Mares were inseminated at five consecutive oestrous periods using fresh ejaculated spermatozoa (Fr-E, n=18), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+, n=12) or fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-, n=9), frozen-thawed ejaculated spermatozoa (Cr-E, n=18), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+, n=18) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-, n=15). Pregnancy examinations were performed 14 days after each ovulation. Pregnancy rates were 55.6% (Fr-E, 10/18), 75% (Fr-SP+, 9/12), 22.2% (Fr-SP-, 2/9), 38.9% (Cr-E, 7/18), 27.8% (Cr-SP+, 5/18) and 6.7% (Cr-SP-, 1/15). Overall pregnancy rates for fresh and frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma were significantly better than for epididymal spermatozoa that had never been exposed to seminal plasma (P<0.05). We conclude that the exposure of stallion epididymal spermatozoa to seminal plasma improves pregnancy rates

Abstract

The use of epididymal stallion spermatozoa for routine artificial insemination can secure easy future use of valuable genetics after unforeseen death or injury of a valuable stallion. The aims of this study were to (1) directly compare pregnancy rates for fresh and frozen-thawed stallion epididymal and ejaculated spermatozoa after conventional artificial insemination and (2) to investigate the effect of seminal plasma on the fertility of epididymal spermatozoa after insemination. Twenty-one mares were randomly assigned to three stallions. Mares were inseminated at five consecutive oestrous periods using fresh ejaculated spermatozoa (Fr-E, n=18), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+, n=12) or fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-, n=9), frozen-thawed ejaculated spermatozoa (Cr-E, n=18), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+, n=18) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-, n=15). Pregnancy examinations were performed 14 days after each ovulation. Pregnancy rates were 55.6% (Fr-E, 10/18), 75% (Fr-SP+, 9/12), 22.2% (Fr-SP-, 2/9), 38.9% (Cr-E, 7/18), 27.8% (Cr-SP+, 5/18) and 6.7% (Cr-SP-, 1/15). Overall pregnancy rates for fresh and frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma were significantly better than for epididymal spermatozoa that had never been exposed to seminal plasma (P<0.05). We conclude that the exposure of stallion epididymal spermatozoa to seminal plasma improves pregnancy rates

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21 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2010
Deposited On:02 Mar 2010 15:03
Last Modified:05 Apr 2016 14:02
Publisher:Elsevier
ISSN:0378-4320
Publisher DOI:https://doi.org/10.1016/j.anireprosci.2009.06.009
PubMed ID:19592182

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