Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-32716

van der Heijden, G W; Derijck, A H A A; Pósfai, E; Giele, M; Pelczar, P; Ramos, L; Wansink, D G; van der Vlag, J; Peters, A H F M; de Boer, P (2007). Chromosome-wide nucleosome replacement and H3.3 incorporation during mammalian meiotic sex chromosome inactivation. Nature Genetics, 39(2):251-258.

[img] PDF - Registered users only
2MB

Abstract

In mammalian males, the first meiotic prophase is characterized by formation of a separate chromatin domain called the sex body. In this domain, the X and Y chromosomes are partially synapsed and transcriptionally silenced, a process termed meiotic sex-chromosome inactivation (MSCI). Likewise, unsynapsed autosomal chromatin present during pachytene is also silenced (meiotic silencing of unsynapsed chromatin, MSUC). Although it is known that MSCI and MSUC are both dependent on histone H2A.X phosphorylation mediated by the kinase ATR, and cause repressive H3 Lys9 dimethylation, the mechanisms underlying silencing are largely unidentified. Here, we demonstrate an extensive replacement of nucleosomes within unsynapsed chromatin, depending on and initiated shortly after induction of MSCI and MSUC. Nucleosomal eviction results in the exclusive incorporation of the H3.3 variant, which to date has primarily been associated with transcriptional activity. Nucleosomal exchange causes loss and subsequent selective reacquisition of specific histone modifications. This process therefore provides a means for epigenetic reprogramming of sex chromatin presumably required for gene silencing in the male mammalian germ line.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Laboratory Animal Science
04 Faculty of Medicine > Institute of Laboratory Animal Science
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2007
Deposited On:03 Mar 2010 12:57
Last Modified:27 Nov 2013 20:42
Publisher:Nature Publishing Group
ISSN:1061-4036
Publisher DOI:10.1038/ng1949
PubMed ID:17237782
Citations:Web of Science®. Times Cited: 107
Google Scholar™
Scopus®. Citation Count: 109

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page