Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-32801
Schneider, T; Gerrits, B; Gassmann, R; Schmid, E; Gessner, M O; Richter, A; Battin, T; Eberl, L; Riedel, K (2010). Proteome analysis of fungal and bacterial involvement in leaf litter decomposition. Proteomics, 10(9):1819-1830.
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Fungi and bacteria are key players in the decomposition of leaf litter, but their individual contributions to the process and their interactions are still poorly known. We combined semi-quantitative proteome analyses (one-dimensional gel electrophoresis, liquid chromatography and tandem mass spectrometry; 1D-PAGE-LC-MS/MS) with qualitative and quantitative analyses of extracellular degradative enzyme activities to unravel the respective roles of a fungus and a bacterium during litter decomposition. Two model organisms, a mesophilic Gram-negative bacterium (Pectobacterium carotovorum) and an ascomycete (Aspergillus nidulans), were grown in both, pure culture and co-culture on minimal medium containing either glucose or beech leaf litter as sole carbon source. P. carotovorum grew best in co-culture with the fungus, whereasgrowth of A. nidulans was significantly reduced when the bacterium was present. This observation suggests that P. carotovorum has only limited capabilities to degrade leaf litter and profits from the degradation products of A. nidulans at the expense of fungal growth. In accordance with this interpretation, our proteome analysis revealed that most of the extracellular biodegradative enzymes (i.e. proteases, pectinases, and cellulases) in the cultures with beech litter were expressed by the fungus, the bacterium producing only low levels of pectinases.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Functional Genomics Center Zurich|
08 University Research Priority Programs > Systems Biology / Functional Genomics
07 Faculty of Science > Institute of Plant Biology
|DDC:||570 Life sciences; biology|
580 Plants (Botany)
610 Medicine & health
|Deposited On:||21 Mar 2010 12:14|
|Last Modified:||28 Nov 2013 02:18|
|Citations:||Web of Science®. Times cited: 17|
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