Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, 5.7.2016, 07:00-08:00

Maintenance work on ZORA and JDB on Tuesday, 5th July, 07h00-08h00. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35233

Al-Sabi, M N S; Deplazes, P; Webster, P; Willesen, J L; Davidson, R K; Kapel, C M O (2010). PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes. Parasitology Research, 107(1):135-40.

View at publisher


The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.


33 citations in Web of Science®
32 citations in Scopus®
Google Scholar™



156 downloads since deposited on 29 Jul 2010
42 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
600 Technology
Date:June 2010
Deposited On:29 Jul 2010 03:42
Last Modified:05 Apr 2016 14:12
Publisher DOI:10.1007/s00436-010-1847-5
PubMed ID:20372926

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page