Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35233
Al-Sabi, M N S; Deplazes, P; Webster, P; Willesen, J L; Davidson, R K; Kapel, C M O (2010). PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes. Parasitology Research, 107(1):135-40.
The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Parasitology|
04 Faculty of Medicine > Institute of Parasitology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||29 Jul 2010 05:42|
|Last Modified:||02 Dec 2013 02:40|
|Citations:||Web of Science®. Times cited: 22|
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